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Ref Type Journal Article
PMID (23674493)
Authors Weigelt B, Warne PH, Lambros MB, Reis-Filho JS, Downward J
Title PI3K pathway dependencies in endometrioid endometrial cancer cell lines.
Journal Clinical cancer research : an official journal of the American Association for Cancer Research
Vol 19
Issue 13
Date 2013 Jul 01
URL
Abstract Text Endometrioid endometrial cancers (EEC) frequently harbor coexisting mutations in phosphoinositide 3-kinase (PI3K) pathway genes, including PTEN, PIK3CA, PIK3R1, and KRAS. We sought to define the genetic determinants of PI3K pathway inhibitor response in EEC cells, and whether PTEN-mutant EEC cell lines rely on p110β signaling for survival.Twenty-four human EEC cell lines were characterized for their mutation profile and activation state of PI3K and mitogen-activated protein kinase (MAPK) signaling pathway proteins. Cells were treated with pan-class I PI3K, p110α, and p110β isoform-specific, allosteric mTOR, mTOR kinase, dual PI3K/mTOR, mitogen-activated protein/extracellular signal-regulated kinase (MEK), and RAF inhibitors. RNA interference (RNAi) was used to assess effects of KRAS silencing in EEC cells.EEC cell lines harboring PIK3CA and PTEN mutations were selectively sensitive to the pan-class I PI3K inhibitor GDC-0941 and allosteric mTOR inhibitor temsirolimus, respectively. Subsets of EEC cells with concurrent PIK3CA and/or PTEN and KRAS mutations were sensitive to PI3K pathway inhibition, and only 2 of 6 KRAS-mutant cell lines showed response to MEK inhibition. KRAS RNAi silencing did not induce apoptosis in KRAS-mutant EEC cells. PTEN-mutant EEC cell lines were resistant to the p110β inhibitors GSK2636771 and AZD6482, and only in combination with the p110α selective inhibitor A66 was a decrease in cell viability observed.Targeted pan-PI3K and mTOR inhibition in EEC cells may be most effective in PIK3CA- and PTEN-mutant tumors, respectively, even in a subset of EECs concurrently harboring KRAS mutations. Inhibition of p110β alone may not be sufficient to sensitize PTEN-mutant EEC cells and combination with other targeted agents may be required.

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Molecular Profile Treatment Approach
PTEN L152P PIK3CB inhibitor
PTEN R130K PIK3CB inhibitor
PTEN F347L PIK3CB inhibitor
PTEN R173C PIK3CB inhibitor
PTEN C218* PIK3CB inhibitor
PTEN D92G PIK3CB inhibitor
PTEN Y68H PIK3CB inhibitor
PTEN N212Kfs*10 PIK3CB inhibitor
PTEN K263* PIK3CB inhibitor
PTEN C105F PIK3CB inhibitor
PTEN A39P PIK3CB inhibitor
PTEN H123D PIK3CB inhibitor
PTEN E106* PIK3CB inhibitor
PTEN D252G PIK3CB inhibitor
PTEN K60fs PIK3CB inhibitor
PTEN L70fs PIK3CB inhibitor
PTEN R335L PIK3CB inhibitor
PTEN R15K PIK3CB inhibitor
PTEN S302Afs*5 PIK3CB inhibitor
PTEN C124G PIK3CB inhibitor
PTEN K254Rfs*42 PIK3CB inhibitor
PTEN A34P PIK3CB inhibitor
PTEN Y27D PIK3CB inhibitor
PTEN E299* PIK3CB inhibitor
PTEN T131fs PIK3CB inhibitor
PTEN E201* PIK3CB inhibitor
PTEN R173P PIK3CB inhibitor
PTEN Y176* PIK3CB inhibitor
PTEN R130* PIK3CB inhibitor
PTEN K322E PIK3CB inhibitor
PTEN K342* PIK3CB inhibitor
PTEN H61D PIK3CB inhibitor
PTEN P95L PIK3CB inhibitor
PTEN G251C PIK3CB inhibitor
PTEN K289Nfs*8 PIK3CB inhibitor
PTEN C211W PIK3CB inhibitor
PTEN Q261* PIK3CB inhibitor
PTEN G129V PIK3CB inhibitor
PTEN E284* PIK3CB inhibitor
PTEN K66fs PIK3CB inhibitor
PTEN G127E PIK3CB inhibitor
PTEN N340fs PIK3CB inhibitor
PTEN S170R PIK3CB inhibitor
PTEN D24G PIK3CB inhibitor
PTEN negative PIK3CB inhibitor
PTEN E291* PIK3CB inhibitor
PTEN K128Q PIK3CB inhibitor
PTEN K125N PIK3CB inhibitor
PTEN G165* PIK3CB inhibitor
PTEN L247_P248del PIK3CB inhibitor
PTEN E284fs PIK3CB inhibitor
PTEN D252Y PIK3CB inhibitor
PTEN Y240* PIK3CB inhibitor
PTEN G165R PIK3CB inhibitor
PTEN I122fs PIK3CB inhibitor
PTEN T319* PIK3CB inhibitor
PTEN A126P PIK3CB inhibitor
PTEN R130L PIK3CB inhibitor
PTEN S385* PIK3CB inhibitor
PTEN L57W PIK3CB inhibitor
PTEN R130G PIK3CB inhibitor
PTEN K125E PIK3CB inhibitor
PTEN C250fs PIK3CB inhibitor
PTEN A121E PIK3CB inhibitor
PTEN C124fs PIK3CB inhibitor
PTEN Q298* PIK3CB inhibitor
PTEN Y155fs PIK3CB inhibitor
PTEN N184Efs*16 PIK3CB inhibitor
PTEN N48K PIK3CB inhibitor
PTEN T321fs PIK3CB inhibitor
PTEN L181P PIK3CB inhibitor
PTEN K102fs PIK3CB inhibitor
PTEN M199del PIK3CB inhibitor
PTEN A126D PIK3CB inhibitor
PTEN H61R PIK3CB inhibitor
PTEN G36E PIK3CB inhibitor
PTEN L57fs PIK3CB inhibitor
PTEN R161G PIK3CB inhibitor
PTEN V275* PIK3CB inhibitor
PTEN K163fs PIK3CB inhibitor
PTEN Y155H PIK3CB inhibitor
PTEN K13Q PIK3CB inhibitor
PTEN M134T PIK3CB inhibitor
PTEN Y155N PIK3CB inhibitor
PTEN F271S PIK3CB inhibitor
PTEN G129D PIK3CB inhibitor
PTEN G251V PIK3CB inhibitor
PTEN A151P PIK3CB inhibitor
PTEN D331fs PIK3CB inhibitor
PTEN Y174N PIK3CB inhibitor
PTEN Y68D PIK3CB inhibitor
PTEN Y68fs PIK3CB inhibitor
PTEN D107Y PIK3CB inhibitor
PTEN T382S PIK3CB inhibitor
PTEN I122L PIK3CB inhibitor
PTEN Y76fs PIK3CB inhibitor
PTEN Y27N PIK3CB inhibitor
PTEN Y68N PIK3CB inhibitor
PTEN D24Y PIK3CB inhibitor
PTEN D268Gfs*30 PIK3CB inhibitor
PTEN K128_R130del PIK3CB inhibitor
PTEN F21A PIK3CB inhibitor
PTEN E7* PIK3CB inhibitor
PTEN K237fs PIK3CB inhibitor
PTEN G129R PIK3CB inhibitor
PTEN L108R PIK3CB inhibitor
PTEN R130fs PIK3CB inhibitor
PTEN Q171* PIK3CB inhibitor
PTEN K13* PIK3CB inhibitor
PTEN V119fs PIK3CB inhibitor
PTEN D116fs PIK3CB inhibitor
PTEN M134I PIK3CB inhibitor
PTEN T348I PIK3CB inhibitor
PTEN D326N PIK3CB inhibitor
PTEN C124N PIK3CB inhibitor
PTEN V133I PIK3CB inhibitor
PTEN Q171E PIK3CB inhibitor
PTEN V317fs PIK3CB inhibitor
PTEN L23F PIK3CB inhibitor
PTEN K6fs PIK3CB inhibitor
PTEN R130P PIK3CB inhibitor
PTEN V166Sfs*14 PIK3CB inhibitor
PTEN T167A PIK3CB inhibitor
PTEN R74Dfs*25 PIK3CB inhibitor
PTEN A126S PIK3CB inhibitor
PTEN K6E PIK3CB inhibitor
PTEN K128T PIK3CB inhibitor
PTEN loss PIK3CB inhibitor
PTEN K125M PIK3CB inhibitor
PTEN C136Y PIK3CB inhibitor
PTEN Y68C PIK3CB inhibitor
PTEN I8Dfs*3 PIK3CB inhibitor
PTEN R15S PIK3CB inhibitor
PTEN E288* PIK3CB inhibitor
PTEN N94I PIK3CB inhibitor
PTEN G20E PIK3CB inhibitor
PTEN T160I PIK3CB inhibitor
PTEN K62R PIK3CB inhibitor
PTEN K125L PIK3CB inhibitor
PTEN G36R PIK3CB inhibitor
PTEN D24V PIK3CB inhibitor
PTEN N12T PIK3CB inhibitor
PTEN G132D PIK3CB inhibitor
PTEN K254fs PIK3CB inhibitor
PTEN T319del PIK3CB inhibitor
PTEN A126G PIK3CB inhibitor
PTEN R15I PIK3CB inhibitor
PTEN N117S PIK3CB inhibitor
PTEN M35R PIK3CB inhibitor
PTEN F241S PIK3CB inhibitor
PTEN R142W PIK3CB inhibitor
PTEN Y27S PIK3CB inhibitor
PTEN G251D PIK3CB inhibitor
PTEN D107V PIK3CB inhibitor
PTEN Y27fs PIK3CB inhibitor
PTEN L345Q PIK3CB inhibitor
PTEN V255A PIK3CB inhibitor
PTEN N292fs PIK3CB inhibitor
PTEN R130Q PIK3CB inhibitor
PTEN Q17* PIK3CB inhibitor
PTEN T131L PIK3CB inhibitor
PTEN V343E PIK3CB inhibitor
PTEN G165E PIK3CB inhibitor
PTEN C136R PIK3CB inhibitor
PTEN K6I PIK3CB inhibitor
PTEN A72fs PIK3CB inhibitor
PTEN Q298fs PIK3CB inhibitor
PTEN R335* PIK3CB inhibitor
PTEN D92V PIK3CB inhibitor
PTEN Y27C PIK3CB inhibitor
PTEN M134L PIK3CB inhibitor
PTEN C124S PIK3CB inhibitor
PTEN L325R PIK3CB inhibitor
PTEN E242* PIK3CB inhibitor
PTEN L265fs PIK3CB inhibitor
PTEN F241fs PIK3CB inhibitor
PTEN I135K PIK3CB inhibitor
PTEN A126fs PIK3CB inhibitor
PTEN R173fs PIK3CB inhibitor
PTEN F90S PIK3CB inhibitor
PTEN R130A PIK3CB inhibitor
PTEN D331Tfs*13 PIK3CB inhibitor
PTEN Y65C PIK3CB inhibitor
PTEN E256* PIK3CB inhibitor
PTEN F56fs PIK3CB inhibitor
PTEN N329fs PIK3CB inhibitor
PTEN F341V PIK3CB inhibitor
PTEN V158fs PIK3CB inhibitor
PTEN inact mut PIK3CB inhibitor
PTEN L70V PIK3CB inhibitor
PTEN S287* PIK3CB inhibitor
PTEN L112R PIK3CB inhibitor
PTEN K13E PIK3CB inhibitor
PTEN V217fs PIK3CB inhibitor
PTEN S10N PIK3CB inhibitor
PTEN L325F PIK3CB inhibitor
PTEN Y155C PIK3CB inhibitor
PTEN R55fs PIK3CB inhibitor
PTEN T167P PIK3CB inhibitor
PTEN L112P PIK3CB inhibitor
PTEN D24N PIK3CB inhibitor
PTEN E73K PIK3CB inhibitor
PTEN L182fs PIK3CB inhibitor
PTEN K267fs PIK3CB inhibitor
PTEN P95S PIK3CB inhibitor
PTEN K254* PIK3CB inhibitor
PTEN C71Y PIK3CB inhibitor
PTEN P38H PIK3CB inhibitor
PTEN L318fs PIK3CB inhibitor
PTEN V290Sfs*8 PIK3CB inhibitor
PTEN D107G PIK3CB inhibitor
PTEN Q245* PIK3CB inhibitor
PTEN G129* PIK3CB inhibitor
PTEN E242fs PIK3CB inhibitor
PTEN Y16fs PIK3CB inhibitor
PTEN S227F PIK3CB inhibitor
PTEN N276S PIK3CB inhibitor
PTEN E288fs PIK3CB inhibitor
PTEN Y65* PIK3CB inhibitor
PTEN N323Kfs*2 PIK3CB inhibitor
PTEN D92A PIK3CB inhibitor
PTEN H123Q PIK3CB inhibitor
PTEN L247Sfs*6 PIK3CB inhibitor
PTEN P339L PIK3CB inhibitor
PTEN I122S PIK3CB inhibitor
PTEN W111fs PIK3CB inhibitor
PTEN T131A PIK3CB inhibitor
PTEN A126V PIK3CB inhibitor
PTEN S170N PIK3CB inhibitor
PTEN F154L PIK3CB inhibitor
PTEN S294fs PIK3CB inhibitor
PTEN K125* PIK3CB inhibitor
PTEN G127N PIK3CB inhibitor
PTEN N63fs PIK3CB inhibitor
PTEN C124R PIK3CB inhibitor
PTEN W274fs PIK3CB inhibitor
PTEN V290fs PIK3CB inhibitor
PTEN P248fs PIK3CB inhibitor
PTEN K260fs PIK3CB inhibitor
PTEN Q214* PIK3CB inhibitor
PTEN G44D PIK3CB inhibitor
PTEN R14fs PIK3CB inhibitor
PTEN T131I PIK3CB inhibitor
PTEN D92H PIK3CB inhibitor
PTEN H123Y PIK3CB inhibitor
PTEN I101F PIK3CB inhibitor
PTEN D92E PIK3CB inhibitor
PTEN H93D PIK3CB inhibitor
PTEN K147* PIK3CB inhibitor
PTEN G129E PIK3CB inhibitor
PTEN L193fs PIK3CB inhibitor
PTEN N323fs PIK3CB inhibitor
PTEN P246fs PIK3CB inhibitor
PTEN del PIK3CB inhibitor
PTEN G293fs PIK3CB inhibitor
PTEN H118P PIK3CB inhibitor
PTEN W111* PIK3CB inhibitor
PTEN V290* PIK3CB inhibitor
PTEN D22E PIK3CB inhibitor
PTEN E157G PIK3CB inhibitor
PTEN V217D PIK3CB inhibitor
PTEN L42R PIK3CB inhibitor
PTEN T167N PIK3CB inhibitor
PTEN L108P PIK3CB inhibitor
PTEN F337fs PIK3CB inhibitor
PTEN G165V PIK3CB inhibitor
PTEN H93Y PIK3CB inhibitor
PTEN M35V PIK3CB inhibitor
PTEN E235* PIK3CB inhibitor
PTEN F56C PIK3CB inhibitor
PTEN C136F PIK3CB inhibitor
PTEN P96Q PIK3CB inhibitor
PTEN T319fs PIK3CB inhibitor
PTEN Q219* PIK3CB inhibitor
PTEN R14G PIK3CB inhibitor
PTEN R233* PIK3CB inhibitor
PTEN S59* PIK3CB inhibitor
PTEN T232A PIK3CB inhibitor
PTEN G143fs PIK3CB inhibitor
PTEN Y138L PIK3CB inhibitor
PTEN G127R PIK3CB inhibitor
PTEN A121P PIK3CB inhibitor
PTEN K260* PIK3CB inhibitor
PTEN H93Q PIK3CB inhibitor
PTEN H118fs PIK3CB inhibitor
PTEN I101T PIK3CB inhibitor
PTEN V54fs PIK3CB inhibitor
PTEN A34D PIK3CB inhibitor
PTEN E285* PIK3CB inhibitor
PTEN Q110* PIK3CB inhibitor
Gene Name Source Synonyms Protein Domains Gene Description Gene Role
Therapy Name Drugs Efficacy Evidence Clinical Trials
AZD6482 AZD6482 7 0
Drug Name Trade Name Synonyms Drug Classes Drug Description
AZD6482 KIN-193|AZD-6482 PIK3CB inhibitor 8 PIK3CD inhibitor 25 AZD6482 inhibits PIK3CB and PIK3CD, preventing the activation of the PI3K signaling pathway and inhibiting tumor cell proliferation, motility, and survival (PMID: 24992874, PMID: 23674493, PMID: 31292159, PMID: 30542720).
Gene Variant Impact Protein Effect Variant Description Associated with drug Resistance
Molecular Profile Indication/Tumor Type Response Type Therapy Name Approval Status Evidence Type Efficacy Evidence References
PTEN mutant endometrial cancer resistant A66 Preclinical Actionable In a preclinical study, endometrioid endometrial cancer cell lines harboring PTEN mutations demonstrated resistance to A66 induced growth inhibition in culture (PMID: 23674493). 23674493
PTEN mutant endometrial cancer sensitive A66 + AZD6482 Preclinical Actionable In a preclinical study, A66 and AZD6482 combination treatment inhibited viability of endometrioid endometrial cancer cell lines harboring PTEN mutations in culture (PMID: 23674493). 23674493
PTEN mutant endometrial cancer sensitive A66 + GSK2636771 Preclinical Actionable In a preclinical study, A66 and GSK2636771 combination treatment inhibited viability of endometrioid endometrial cancer cell lines harboring PTEN mutations in culture (PMID: 23674493). 23674493
PTEN loss breast cancer sensitive GSK2636771 Preclinical Actionable In a preclinical study, GSK2636771 inhibited viability of breast cancer cell lines harboring PTEN deficiency in culture (PMID: 23674493). 23674493
PTEN loss prostate cancer sensitive GSK2636771 Preclinical Actionable In a preclinical study, GSK2636771 inhibited viability of prostate cancer cell lines harboring PTEN deficiency in culture (PMID: 23674493). 23674493
PTEN mutant endometrial cancer resistant AZD6482 Preclinical Actionable In a preclinical study, endometrioid endometrial cancer cell lines harboring PTEN mutations demonstrated resistance to AZD6482 induced growth inhibition in culture (PMID: 23674493). 23674493
PTEN mutant endometrial cancer resistant GSK2636771 Preclinical Actionable In a preclinical study, endometrioid endometrial cancer cell lines harboring PTEN mutations demonstrated resistance to GSK2636771 induced growth inhibition in culture (PMID: 23674493). 23674493
PTEN loss breast cancer sensitive AZD6482 Preclinical Actionable In a preclinical study, AZD6482 inhibited viability of breast cancer cell lines harboring PTEN deficiency in culture (PMID: 23674493). 23674493
PTEN loss prostate cancer sensitive AZD6482 Preclinical Actionable In a preclinical study, AZD6482 inhibited viability of prostate cancer cell lines harboring PTEN deficiency in culture (PMID: 23674493). 23674493
PIK3CA mutant endometrial cancer sensitive Pictilisib Preclinical Actionable In a preclinical study, endometrioid endometrial cancer cell lines harboring PIK3CA mutations demonstrated increased sensitivity to GDC-0941 (Pictilisib) induced growth inhibition comparing to PIK3CA wild-type cells in culture (PMID: 23674493). 23674493
PTEN mutant endometrial cancer resistant TGX-221 Preclinical Actionable In a preclinical study, endometrioid endometrial cancer cell lines harboring PTEN mutations demonstrated resistance to TGX-221 induced growth inhibition in culture (PMID: 23674493). 23674493