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Gene Symbol ALK
Synonyms ALK1 | CD246 | NBLST3
Gene Description ALK, anaplastic lymphoma kinase, is a receptor in the insulin receptor superfamily and is a key regulator of neuronal development (PMID: 21502284) and also promotes cell proliferation through activation of MAPK and PI3K signaling pathways (PMID: 27573755). Alk activating mutations, rearrangements, and fusions have been identified in various cancers (PMID: 22649787), including EML4-ALK in non-small cell lung cancer (PMID: 30108712, PMID: 30194140), and a number of mutations confer resistance in the context of Alk fusions (PMID: 25749034, PMID: 21948233).

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Variant Impact Protein Effect Variant Description Associated with drug Resistance
A1047T missense unknown ALK A1047T lies within the transmembrane domain of the Alk protein (UniProt.org). A1047T has been identified in sequencing studies (PMID: 28524162), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
A1200V missense no effect - predicted ALK A1200V lies within the protein kinase domain of the Alk protein (UniProt.org). A1200V demonstrates kinase activity comparable to wild-type Alk in culture (PMID: 25517749), and therefore, is predicted to have no effect on Alk protein function.
A1200_G1201delinsW indel unknown ALK A1200_G1201delinsW results in deletion of an alanine (A) and a glycine (G) from aa 1200 to aa 1201 in the protein kinase domain of the Alk protein, combined with the insertion of a tryptophan (W) at the same site (UniProt.org). A1200_G1201delinsW has been associated with secondary drug resistance to some ALK inhibitors (PMID: 34548910), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jun 2022). Y
A1251T missense loss of function - predicted ALK A1251T lies within the protein kinase domain of the Alk protein (UniProt.org). A1251T results in loss of kinase activity in an in vitro assay and is not transforming in cultured cells (PMID: 33674381), and therefore, is predicted to lead to a loss of Alk protein function.
A1252V missense no effect - predicted ALK A1252V lies within the cytoplasmic domain of the Alk protein (UniProt.org). A1252V does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
A1266D missense unknown ALK A1266D lies within the protein kinase domain of the Alk protein (UniProt.org). A1266D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022.
A1266V missense no effect - predicted ALK A1266V lies within the protein kinase domain of the Alk protein (UniProt.org). A1266V has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
A195V missense no effect - predicted ALK A195V lies within the extracellular domain of the Alk protein (UniProt.org). A195V has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
A348D missense gain of function ALK A348D lies within the MAM domain 1 of the Alk protein (UniProt.org). A384D confers a gain of function to the Alk protein as demonstrated by increased cell proliferation in the absence of growth factor and colony formation in culture (PMID: 26032424).
A585T missense no effect - predicted ALK A585T lies within the MAM domain 2 of the Alk protein (UniProt.org). A585T has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
act mut unknown gain of function ALK act mut indicates that this variant results in a gain of function in the Alk protein. However, the specific amino acid change has not been identified.
ALK - ERLEC1 fusion unknown ALK-ERLEC1 results from the fusion of ALK and ERLEC1 (PMID: 33419583). ALK-ERLEC1 has been identified in lung adenocarcinoma (PMID: 33419583), but has not been biochemically characterized and therefore, the effect on protein function is unknown (PubMed, Jul 2022).
ALK - HLA-DRB1 fusion unknown ALK-HLA-DRB1 results from the fusion of ALK and HLA-DRB1 (PMID: 35280798). ALK-HLA-DRB1 has been identified in lung adenocarcinoma (PMID: 35280798), but has not been biochemically characterized and therefore, the effect on protein function is unknown (PubMed, Sep 2022).
ALK - SSH2 fusion unknown ALK-SSH2 results from the fusion of ALK and SSH2 (PMID: 35144623). ALK-SSH2 has been identified in lung adenocarcinoma (PMID: 35144623), but has not been biochemically characterized and therefore, the effect on protein function is unknown (PubMed, Jul 2022).
amp none no effect ALK amplification indicates an increased number of copies of the ALK gene. However, the mechanism causing the increase is unspecified.
C1021V missense no effect - predicted ALK C1021V lies within the extracellular domain of the Alk protein (UniProt.org). C1021V does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
C1097A missense unknown ALK C1097A lies within the cytoplasmic domain of the Alk protein (UniProt.org). C1097A is not activating in an in vitro assay and is weakly transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
C1156F missense unknown ALK C1156F lies within the protein kinase domain of the Alk protein (UniProt.org). C1156F has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034, PMID: 30322862), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
C1156Y missense unknown ALK C1156Y lies within the protein kinase domain of the Alk protein (UniProt.org). C1156Y has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 21613408, PMID: 20979473, PMID: 27490033, PMID: 27045755), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
C928Lfs*20 frameshift loss of function - predicted ALK C928Lfs*20 indicates a shift in the reading frame starting at amino acid 928 and terminating 20 residues downstream causing a premature truncation of the 1620 amino acid Alk protein (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), C928Lfs*20 is predicted to lead to a loss of Alk protein function.
C990R missense unknown ALK C990R lies within the extracellular domain of the Alk protein (UniProt.org). C990R increased cell proliferation and cell viability compared to wild-type Alk in one of two cell lines in culture (PMID: 29533785), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
D1091N missense unknown ALK D1091N lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1091N was observed to increase ERK activation in the presence of agonist antibodies (PMID: 23104988), but is unable to transform cultured cells (PMID: 23104988), and therefore, its effect on Alk protein function is unknown.
D1163N missense gain of function - predicted ALK D1163N lies within the protein kinase domain of the Alk protein (UniProt.org). D1163N is not transforming in cultured cells but is activating in an in vitro assay (PMID: 33674381), and therefore, is predicted to lead to a gain of Alk protein function.
D1203fs frameshift loss of function - predicted ALK D1203fs results in a change in the amino acid sequence of the Alk protein beginning at aa 1203 of 1620, likely resulting in premature truncation of the functional protein (UniProt.org). Due to the loss of the ATP binding site (UniProt.org), D1203fs is predicted to lead to a loss of Alk protein function.
D1203N missense unknown ALK D1203N lies within the protein kinase domain of the Alk protein (UniProt.org). D1203N has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 25421750, PMID: 27432227, PMID: 28434515), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
D1249fs frameshift loss of function - predicted ALK D1249fs results in a change in the amino acid sequence of the Alk protein beginning at aa 1249 of 1620, likely resulting in premature truncation of the functional protein (UniProt.org). Due to the loss of the ATP binding site (UniProt.org), D1249fs is predicted to lead to a loss of Alk protein function.
D1270G missense loss of function - predicted ALK D1270G lies within the protein kinase domain of the Alk protein (UniProt.org). D1270G results in loss of kinase activity in in vitro assays and is not transforming in culture (PMID: 33674381, PMID: 25517749), and therefore, is predicted to lead to a loss of Alk protein function.
D1311N missense no effect - predicted ALK D1311N lies within the protein kinase domain of the Alk protein (UniProt.org). D1311N does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
D1349H missense unknown ALK D1349H lies within the protein kinase domain of the Alk protein (UniProt.org). D1349H is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
D1529E missense no effect - predicted ALK D1529E lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1529E has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
D1529G missense unknown ALK D1529G lies within the cytoplasmic domain of the Alk protein (UniProt.org). D1529G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
D94N missense unknown ALK D94N lies within the extracellular domain of the Alk protein (UniProt.org). D94N has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
E1129V missense unknown ALK E1129V lies within the protein kinase domain of the Alk protein (UniProt.org). E1129V has been identified in the scientific literature (PMID: 34890832, PMID: 36093526, PMID: 33161228), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Sep 2022).
E1154K missense unknown ALK E1154K lies within the protein kinase domain of the Alk protein (UniProt.org). E1154K has been identified in the scientific literature (PMID: 31585938), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
E1161A missense unknown ALK E1161A lies within the protein kinase domain of the Alk protein (UniProt.org). E1161A is not activating in an in vitro assay and is weakly transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
E1210K missense unknown ALK E1210K lies within the protein kinase domain of the Alk protein (UniProt.org). E1210K has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 29650534, PMID: 25914136, PMID: 27432227), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
E1242K missense unknown ALK E1242K lies within the protein kinase domain of the Alk protein (UniProt.org). E1242K is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
E1303K missense unknown ALK E1303K lies within the protein kinase domain of the Alk protein (UniProt.org). E1303K has been identified in the scientific literature (PMID: 29978950), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
E1400D missense unknown ALK E1400D lies within the cytoplasmic domain of the Alk protein (UniProt.org). E1400D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
E1407K missense unknown ALK E1407K lies within the cytoplasmic domain of the Alk protein (UniProt.org). E1407K has been identified in the scientific literature (PMID: 29290262, PMID: 35042152), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Nov 2022).
E310D missense unknown ALK E310D lies within the MAM domain 1 of the Alk protein (UniProt.org). E310D has been identified in sequencing studies (PMID: 28915720), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jun 2022).
E717K missense unknown ALK E717K lies within the extracellular domain of the Alk protein (UniProt.org). E717K has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
E862D missense unknown ALK E862D lies within the extracellular domain of the Alk protein (UniProt.org). E862D has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
E994K missense no effect - predicted ALK E994K lies within the extracellular domain of the Alk protein (UniProt.org). E994K has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
F1098V missense unknown ALK F1098V lies within the cytoplasmic domain of the Alk protein (UniProt.org). F1098V is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
F1174C missense gain of function ALK F1174C lies within the protein kinase domain of the Alk protein (UniProt.org). F1174C confers a gain of function to Alk, as indicated by constitutive Alk phosphorylation in cell culture (PMID: 24509625, PMID: 22072639).
F1174I missense gain of function ALK F1174I lies within the protein kinase domain of the Alk protein (UniProt.org). F1174I results in ligand-independent phosphorylation of the Alk protein, activation of Erk, Stat3 pathways, and is transforming in cell culture (PMID: 23104988).
F1174L missense gain of function ALK F1174L lies within the protein kinase domain of the Alk protein (UniProt.org). F1174L confers a gain of function to the Alk protein as indicated by transformation activity and increased cell proliferation in culture (PMID: 18923525, PMID: 29533785, PMID: 29907598), and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 21030459, PMID: 31452835). Y
F1174S missense gain of function ALK F1174S lies within the protein kinase domain of the Alk protein (UniProt.org). F1174S results in ligand-independent activation of the Alk protein and increased Erk phosphorylation in culture (PMID: 21059859), activation in an in vitro assay (PMID: 33674381), and transformation of cells in culture (PMID: 21059859, PMID: 33674381).
F1174V missense gain of function - predicted ALK F1174V lies within the protein kinase domain of the Alk protein (UniProt.org). F1174V is predicted to confer a gain of function to the Alk protein as indicated by constitutive activation of the Alk protein in cell culture (PMID: 21242967), and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 24675041). Y
F1174X missense unknown ALK F1174X indicates any Alk missense mutation that results in replacement of the phenylalanine (F) at amino acid 1174 by a different amino acid.
F1245C missense gain of function ALK F1245C lies within the protein kinase domain of the Alk protein (UniProt.org). F1245C results in increased downstream signaling and is transforming in cell culture (PMID: 21838707, PMID: 25517749). Y
F1245I missense unknown ALK F1245I lies within the protein kinase domain of the Alk protein (UniProt.org). F1245I has been identified in the scientific literature (PMID: 30778092), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
F1245L missense unknown ALK F1245L lies within the protein kinase domain of the Alk protein (UniProt.org). F1245L has been identified in the scientific literature (PMID: 30867766, PMID: 18923524), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
F1245Q missense unknown ALK F1245Q lies within the protein kinase domain of the Alk protein (UniProt.org). F1245Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
F1245V missense gain of function ALK F1245V lies within the protein kinase domain of the Alk protein (UniProt.org). F1245V confers a gain of function to the Alk protein, as it results in increased Alk kinase activity in vitro and leads to transformation activity in cell culture (PMID: 25517749, PMID: 33674381, PMID: 25517749).
F1245X missense unknown ALK F1245X indicates any Alk missense mutation that results in replacement of the phenylalanine (F) at amino acid 1245 by a different amino acid.
F1271L missense unknown ALK F1271L lies within the protein kinase domain of the Alk protein (UniProt.org). F1271L is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
F457L missense no effect - predicted ALK F457L lies within the LDL-receptor class A domain of the Alk protein (UniProt.org). F457L has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
F856S missense gain of function ALK F856S lies within the extracellular domain of the Alk protein (UniProt.org). F856S confers a gain of function to the Alk protein as demonstrated by increased cell proliferation in the absence of growth factor and colony formation in culture (PMID: 26032424).
fusion fusion unknown ALK fusion indicates a fusion of the ALK gene, but the fusion partner is unknown.
G1123D missense unknown ALK G1123D lies within the protein kinase domain of the Alk protein (UniProt.org). G1123D has been demonstrated to confer drug resistance in cell culture (PMID: 21948233), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1123S missense unknown ALK G1123S lies within the protein kinase domain of the Alk protein (UniProt.org). G1123S has been demonstrated to confer drug resistance in culture (PMID: 26134233, PMID: 21948233), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1128A missense gain of function ALK G1128A lies within the protein kinase domain of the Alk protein (UniProt.org). G1128A confers a gain of function to the Alk protein as demonstrated by increased activation in in vitro assays (PMID: 21838707, PMID: 33674381, PMID: 25517749) and transformation in cultured cells (PMID: 21838707, PMID: 33674381, PMID: 25517749).
G1128S missense unknown ALK G1128S lies within the protein kinase domain of the Alk protein (UniProt.org). G1128S has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1137R missense no effect - predicted ALK G1137R lies within the protein kinase domain of the Alk protein (UniProt.org). G1137R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
G1201E missense gain of function ALK G1201E lies within the protein kinase domain of the Alk protein (UniProt.org). G1201E confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity and downstream Pi3k and Mapk pathway activation in culture (PMID: 21596819).
G1201R missense unknown ALK G1201R lies within the protein kinase domain of the Alk protein (UniProt.org). G1201R is transforming (PMID: 33674381), but results in impaired kinase activity in cultured cells (PMID: 33674381, PMID: 28030793), and therefore, its effect on Alk protein function is unknown.
G1202del deletion unknown ALK G1202del results in the deletion of an amino acid in the protein kinase domain of the Alk protein at amino acid 1202 (UniProt.org). G1202del has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 27432227), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1202K missense unknown ALK G1202K lies within the protein kinase domain of the Alk protein (UniProt.org). G1202K has been identified in the scientific literature (PMID: 33790576), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
G1202L missense unknown ALK G1202L lies within the protein kinase domain of the Alk protein (UniProt.org). G1202L has been identified in the scientific literature (PMID: 33380260), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2022).
G1202R missense unknown ALK G1202R lies within the protein kinase domain of the Alk protein (UniProt.org). G1202R has been demonstrated to confer drug resistance in the context of ALK fusions (PMID: 22277784, PMID: 24736079), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1202X missense unknown ALK G1202X indicates any ALK missense mutation that results in replacement of the glycine (G) at amino acid 1202 by a different amino acid.
G1269A missense unknown ALK G1269A lies within the protein kinase domain of the Alk protein (UniProt.org). G1269A has been demonstrated to occur as a secondary resistance mutation in the context of ALK rearrangement (PMID: 30675302, PMID: 28434515, PMID: 29872693), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
G1269E missense unknown ALK G1269E lies within the protein kinase domain of the Alk protein (UniProt.org). G1269E has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
G1269S missense gain of function - predicted ALK G1269S lies within the protein kinase domain of the Alk protein (UniProt.org). G1269S is predicted to confer a gain of function on the Alk protein as indicated by increased phosphorylation of Alk (PMID: 21948233) and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK (PMID: 21948233, PMID: 22034911). Y
G1286R missense unknown ALK G1286R lies within the protein kinase domain of the Alk protein (UniProt.org). G1286R is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
G1552R missense no effect - predicted ALK G1552R lies within the cytoplasmic domain of the Alk protein (UniProt.org). G1552R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
G689V missense unknown ALK G689V lies within the extracellular domain of the Alk protein (UniProt.org). G689V has been identified in sequencing studies (PMID: 26960398), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
G746C missense unknown ALK G746C lies within the extracellular domain of the Alk protein (UniProt.org). G746C has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
G875R missense no effect - predicted ALK G875R lies within the extracellular domain of the Alk protein (UniProt.org). G875R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in culture cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
G922R missense unknown ALK G922R lies within the extracellular domain of the Alk protein (UniProt.org). G922R has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
H1030P missense unknown ALK H1030P lies within the extracellular domain of the ALK protein (UniProt.org). H1030P has been identified in sequencing studies (PMID: 25275298), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
H354Q missense no effect - predicted ALK H354Q lies within the MAM domain 1 of the Alk protein (UniProt.org). H354Q has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cell culture (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
I1170N missense gain of function ALK I1170N lies within the protein kinase domain of the Alk protein (UniProt.org). I1170N confers a gain of function to Alk as demonstrated by increased kinase activity in in vitro assays and transformation in cultured cells (PMID: 33674381, PMID: 25517749).
I1170S missense gain of function ALK I1170S lies within the protein kinase domain of the Alk protein (UniProt.org). I1170S confers a gain of function to Alk as demonstrated by increased kinase activity in in vitro assays and transformation in cultured cells (PMID: 33674381, PMID: 25517749).
I1170V missense gain of function - predicted ALK I1170V lies within the protein kinase domain of the Alk protein (UniProt.org). I1170V is not transforming in cultured cells but is activating in an in vitro assay (PMID: 33674381), and therefore, is predicted to lead to a gain of Alk protein function.
I1171N missense gain of function ALK I1171N lies within the protein kinase domain of the Alk protein (UniProt.org). I1171N results in increased ligand-independent Alk phosphorylation and activation of the Stat pathway in culture (PMID: 23239810, PMID: 21838707), activation in in vitro assays (PMID: 33674381, PMID: 25517749), is transforming in cell culture (PMID: 23239810, (PMID: 33674381, PMID: 25517749), and has been demonstrated to occur as a secondary resistance mutation in the context of ALK fusions (PMID: 27565911). Y
I1171S missense unknown ALK I1171S lies within the protein kinase domain of the Alk protein (UniProt.org). I1171S has been demonstrated to confer drug resistance in the context of ALK fusions in culture (PMID: 27009859, PMID: 25393796, PMID: 26464158), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
I1171T missense gain of function ALK I1171T lies within the protein kinase domain of the Alk protein (UniProt.org). I1171T confers a gain of function on the Alk protein as indicated by ligand-independent autophosphorylation, activation of Erk1/2, and cell transformation (PMID: 29907598), and has been demonstrated to confer drug resistance in the context of ALK fusions in culture (PMID: 27009859). Y
I1171X missense unknown ALK I1171X indicates any Alk missense mutation that results in replacement of the isoleucine (I) at amino acid 1171 by a different amino acid.
I1179V missense unknown ALK I1179V lies within the protein kinase domain of the Alk protein (UniProt.org). I1179V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
I1183T missense unknown ALK I1183T lies within the protein kinase domain of the Alk protein (UniProt.org). I1183T is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
I1250T missense loss of function ALK I1250T lies within the protein kinase domain of the Alk protein (UniProt.org). I1250T confers a loss of function on Alk as indicated by loss of kinase activity and loss of the ability to activate downstream signaling pathways in cell culture (PMID: 21804922).
I1268V missense unknown ALK I1268V lies within the protein kinase domain of the Alk protein (UniProt.org). I1268V has been identified in the scientific literature (PMID: 31585938), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
I1461V missense unknown ALK I1461V lies within the cytoplasmic domain of the Alk protein (UniProt.org). I1461V has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
inact mut unknown loss of function ALK inact mut indicates that this variant results in a loss of function of the Alk protein. However, the specific amino acid change has not been identified.
K1062M missense gain of function ALK K1062M lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1062M confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity (PMID: 28199182) and increased AKT and ERK phosphorylation in in vitro assays (PMID: 21596819).
K1491R missense no effect - predicted ALK K1491R lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1491R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
K1518N missense no effect - predicted ALK K1518N lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1518N does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
K1525E missense no effect - predicted ALK K1525E lies within the cytoplasmic domain of the Alk protein (UniProt.org). K1525E does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
K894T missense unknown ALK K894T lies within the extracellular domain of the Alk protein (UniProt.org). K894T has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
L1122V missense unknown ALK L1122V lies within the protein kinase domain of the Alk protein (UniProt.org). L1122V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 29650534, PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1152M missense unknown ALK L1152M lies within the protein kinase domain of the Alk protein (UniProt.org). L1152M has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
L1152P missense unknown ALK L1152P lies within the protein kinase domain of the Alk protein (UniProt.org). L1152P has been demonstrated to confer drug resistance in the context of ALK rearrangement in culture (PMID: 24675041), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Sep 2022). Y
L1152R missense unknown ALK L1152R lies within the protein kinase domain of the Alk protein (UniProt.org). L1152R has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 21791641, PMID: 27091190), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1152V missense unknown ALK L1152V lies within the protein kinase domain of the Alk protein (UniProt.org). L1152V is associated with decreased ALK inhibitor sensitivity in the context of an ALK fusion in culture (PMID: 22034911), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Sep 2022).
L1196M missense gain of function ALK L1196M lies within the protein kinase domain of the Alk protein (UniProt.org). L1196M results in increased Alk kinase activity (PMID: 30258533), modest Alk autophosphorylation, and transformation in cell culture (PMID: 25517749), and confers resistance to Alk inhibitors in the context of ALK rearrangements in culture and in vivo (PMID: 21613408, PMID: 25421750, PMID: 31452835). Y
L1196Q missense unknown ALK L1196Q lies within the protein kinase domain of the Alk protein (UniProt.org). L1196Q has been demonstrated to confer resistance to Alk inhibitors in the context of ALK rearrangements (PMID: 23239810, PMID: 25749034, PMID: 33209633), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022). Y
L1196X missense unknown ALK L1196X indicates any Alk missense mutation that results in replacement of the leucine (L) at amino acid 1196 by a different amino acid.
L1198F missense gain of function ALK L1198F lies within the protein kinase domain of the Alk protein (UniProt.org). L1198F confers a gain of function to the Alk protein as demonstrated by increased Alk kinase activity and downstream Pi3k and Mapk pathway activation (PMID: 21596819).
L1198H missense unknown ALK L1198H lies within the protein kinase domain of the Alk protein (UniProt.org). L1198H has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1198P missense gain of function - predicted ALK L1198P lies within the protein kinase domain of the Alk protein (UniProt.org). L1198P is predicted to confer a gain of function on the Alk protein as indicated by increased phosphorylation of Alk and has been demonstrated to confer resistance to Alk inhibitors in the context of EML4-ALK in culture (PMID: 21948233). Y
L1198V missense unknown ALK L1198V lies within the protein kinase domain of the Alk protein (UniProt.org). L1198V has been demonstrated to confer resistance to ALK inhibitors in the context of EML4-ALK and ALK F1174L (PMID: 29636358), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1198X missense unknown ALK L1198X indicates any Alk missense mutation that results in replacement of the leucine (L) at amino acid 1198 by a different amino acid.
L1204F missense unknown ALK L1204F lies within the protein kinase domain of the Alk protein (UniProt.org). L1204F is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
L1204V missense unknown ALK L1204V lies within the protein kinase domain of the Alk protein (UniProt.org). L1204V has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 29650534), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1256F missense unknown ALK L1256F lies within the protein kinase domain of the Alk protein (UniProt.org). L1256F demonstrates transforming and tumorigenic activity and confers drug resistance in the context of ALK fusions (PMID: 29650534, PMID: 30662002), but has not been individually characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
L1319V missense unknown ALK L1319V lies within the protein kinase domain of the Alk protein (UniProt.org). L1319V has been identified in the scientific literature (PMID: 33776709), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Sep 2022).
L560F missense no effect - predicted ALK L560F lies within the MAM domain 2 of the Alk protein (UniProt.org). L560F has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
L868Q missense unknown ALK L868Q lies within the extracellular domain of the Alk protein (UniProt.org). L868Q has not been characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
M1166R missense gain of function ALK M1166R lies within the protein kinase domain of the Alk protein (UniProt.org). M1166R confers a gain of function to the Alk protein, as indicated by ligand-independent activation of the Alk protein (PMID: 33674381, PMID: 25517749, PMID: 23104988), increased Erk and Stat3 phosphorylation in cell culture (PMID: 23104988), and transformation of cultured cells (PMID: 33674381, PMID: 25517749).
M1166V missense unknown ALK M1166V lies within the protein kinase domain of the Alk protein (UniProt.org). M1166V has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
M1478T missense unknown ALK M1478T lies within the cytoplasmic domain of the Alk protein (UniProt.org). M1478T has been identified in sequencing studies (PMID: 27852271), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
M301I missense no effect - predicted ALK M301I lies within the MAM domain 1 of the Alk protein (UniProt.org). M301I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in culture cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
M552I missense no effect - predicted ALK M552I lies within the MAM domain 2 of the Alk protein (UniProt.org). M552I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
mutant unknown unknown ALK mutant indicates an unspecified mutation in the ALK gene.
N1178H missense unknown ALK N1178H lies within the protein kinase domain of the Alk protein (UniProt.org). N1178H has been demonstrated to occur as a secondary drug resistance mutation in the context of NPM1-ALK (PMID: 25749034), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
negative unknown loss of function ALK negative indicates a lack of expression of the ALK mRNA and/or protein.
over exp none no effect ALK over exp indicates an over expression of the Alk protein and/or mRNA. However, the mechanism causing the over expression is unspecified.
P1112Q missense unknown ALK P1112Q lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1112Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P1139S missense unknown ALK P1139S lies within the protein kinase domain of the Alk protein (UniProt.org). P1139S has been associated with decreased sensitivity to some ALK inhibitors in the context of NPM1-ALK (PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
P1213C missense unknown ALK P1213C lies within the protein kinase domain of the Alk protein (UniProt.org). P1213C is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
P1298S missense unknown ALK P1298S lies within the protein kinase domain of the Alk protein (UniProt.org). P1298S has been identified in sequencing studies (PMID: 24755471), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P1543S missense unknown ALK P1543S lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1543S has been identified in sequencing studies (PMID: 24755471), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
P157S missense unknown ALK P157S lies within the extracellular domain of the Alk protein (Uniprot.org). P157S has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P1599S missense unknown ALK P1599S lies within the cytoplasmic domain of the Alk protein (UniProt.org). P1599S has been identified in sequencing studies (PMID: 29684080), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P279L missense unknown ALK P279L lies within the MAM domain 1 of the Alk protein (UniProt.org). P279L has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P336S missense no effect - predicted ALK P336S lies within the MAM domain 1 of the Alk protein (UniProt.org). P336S has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
P36S missense unknown ALK P36S lies within the extracellular domain of the Alk protein (UniProt.org). P36S has been identified in sequencing studies (PMID: 20579941), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
P40L missense unknown ALK P40L lies within the extracellular domain of the Alk protein (UniProt.org). P40L has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
P858S missense no effect - predicted ALK P858S lies within the extracellular domain of the Alk protein (UniProt.org). P858S has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
positive unknown unknown ALK positive indicates the presence of the ALK gene, mRNA, and/or protein. ALK positive has been used alternatively to refer to presence of an ALK fusion or rearrangement. For related data, refer to ALK fusion or ALK rearrange in CKB.
Q1146K missense unknown ALK Q1146K lies within the protein kinase domain of the Alk protein (UniProt.org). Q1146K has been identified in the scientific literature (PMID: 34687488), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2022).
Q1146P missense unknown ALK Q1146P lies within the protein kinase domain of the Alk protein (UniProt.org). Q1146P has been identified in the scientific literature (PMID: 31894386), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2022).
Q1188_L1190del deletion unknown ALK Q1188_L1190del results in the deletion of three amino acids in the protein kinase domain of the Alk protein from amino acids 1188 to 1190 (UniProt.org). Q1188_L1190del has been identified in the scientific literature (PMID: 33887694), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Nov 2022).
R1113Q missense unknown ALK R1113Q lies within the cytoplasmic domain of the Alk protein (UniProt.org). R1113Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
R1181H missense unknown ALK R1181H lies within the protein kinase domain of the Alk protein (UniProt.org). R1181H has been identified in the scientific literature (PMID: 35441911), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Nov 2022).
R1192P missense gain of function ALK R1192P lies within the protein kinase domain of the Alk protein (UniProt.org). R1192P confers a gain of function to the Alk protein as demonstrated by increased downstream signalling (PMID: 21838707), activation in in vitro assays (PMID: 33674381, PMID: 25517749), and transformation of cultured cells (PMID: 21838707, PMID: 33674381, PMID: 25517749).
R1192Q missense no effect - predicted ALK R1192Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1192Q does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
R1209Q missense unknown ALK R1209Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1209Q leads to decreased cell proliferation and cell viability compared to wild-type Alk in one of two cell lines in culture (PMID: 29533785), but has not been biochemically characterized, and therefore, its effect on Alk protein function is unknown.
R1212C missense unknown ALK R1212C lies within the protein kinase domain of the Alk protein (UniProt.org). R1212C is not activating in an in vitro assay and is weakly transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
R1212H missense unknown ALK R1212H lies within the protein kinase domain of the Alk protein (UniProt.org). R1212H has been identified in sequencing studies (PMID: 21499247, PMID: 28912153, PMID: 28581676), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
R1231Q missense unknown ALK R1231Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1231Q is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
R1275L missense unknown ALK R1275L lies within the protein kinase domain of the Alk protein (UniProt.org). R1275L has been identified in the scientific literature (PMID: 27888620), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
R1275Q missense gain of function ALK R1275Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1275Q confers a gain of function to the Alk protein as demonstrated by transformation activity in cell culture and increased downstream signalling in in vitro assays (PMID: 21838707, PMID: 29533785).
R1275X missense unknown ALK R1275X indicates any Alk missense mutation that results in replacement of the arginine (R) at amino acid 1275 by a different amino acid.
R1279Q missense unknown ALK R1279Q lies within the protein kinase domain of the Alk protein (UniProt.org). R1279Q is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
R133H missense no effect - predicted ALK R133H lies within the extracellular domain of the Alk protein (UniProt.org). R133H has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
R284K missense unknown ALK R284K lies within MAM domain 1 of the Alk protein (UniProt.org). R284K has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
R291C missense unknown ALK R291C lies within MAM domain 1 of the Alk protein (UniProt.org). R291C has been identified in sequencing studies (PMID: 24755471), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
R311H missense unknown ALK R311H lies within MAM domain 1 of the Alk protein (UniProt.org). R311H has been identified in sequencing studies (PMID: 30410351, PMID: 23202128, PMID: 25344691), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
R395H missense unknown ALK R395H lies within MAM domain 1 of the Alk protein (UniProt.org). R395H has been identified in sequencing studies (PMID: 22877736), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022).
R401* nonsense loss of function - predicted ALK R401* results in a premature truncation of the Alk protein at amino acid 401 of 1620 (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), R401* is predicted to lead to a loss of Alk protein function.
R401L missense unknown ALK R401L lies within MAM domain 1 of the Alk protein (UniProt.org). R401L has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
R401Q missense no effect - predicted ALK R401Q lies within the MAM domain 1 of the Alk protein (UniProt.org). R401Q has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted have no effect on Alk protein function.
R412C missense no effect - predicted ALK R412C lies within the MAM domain 1 of the Alk protein (UniProt.org). R412C does not result in Alk autophosphorylation and is not transforming in culture (PMID: 22086496), and therefore, is predicted to have no effect on Alk protein function.
R753Q missense unknown ALK R753Q lies within the extracellular domain of the Alk protein (UniProt.org). R753Q has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
rearrange unknown unknown ALK rearrangement indicates an unspecified rearrangement of the ALK gene.
S1053F missense unknown ALK S1053F lies within the transmembrane domain of the Alk protein (UniProt.org). S1053F has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
S1206C missense unknown ALK S1206C lies within the protein kinase domain of the Alk protein (UniProt.org). S1206C has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK compound mutations (PMID: 25421750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
S1206F missense unknown ALK S1206F lies within the protein kinase domain of the Alk protein (UniProt.org). S1206F has been identified in the scientific literature (PMID: 27565908, PMID: 31712133), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
S1206R missense unknown ALK S1206R lies within the protein kinase domain of the Alk protein (UniProt.org). S1206R has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 22034911), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Sep 2022). Y
S1206Y missense unknown ALK S1206Y lies within the protein kinase domain of the Alk protein (UniProt.org). S1206Y has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK rearrangements (PMID: 22277784, PMID: 24675041, PMID: 25727400), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
S529Y missense no effect - predicted ALK S529Y lies within the MAM domain 2 of the Alk protein (UniProt.org). S529Y has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
S711R missense no effect - predicted ALK S711R lies within the extracellular domain of the Alk protein (UniProt.org). S711R has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
S865P missense no effect - predicted ALK S865P lies within the extracellular domain of the Alk protein (UniProt.org). S865P has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
T1151dup duplication unknown ALK T1151dup indicates the insertion of the duplicate amino acid, threonine (T)-1151, in the protein kinase domain of the Alk protein (UniProt.org). T1151dup has been demonstrated to occur as a secondary drug resistance mutation in the context of EML4-ALK (PMID: 22277784, PMID: 20695522), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022). Y
T1151K missense unknown ALK T1151K lies within the protein kinase domain and inhibitor binding region of the Alk protein (UniProt.org). T1151K has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 28676215, PMID: 30519133), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Jul 2022). Y
T1151M missense gain of function - predicted ALK T1151M lies within the protein kinase domain and inhibitor binding region of the Alk protein (UniProt.org). T1151M has been demonstrated to occur as a secondary drug resistance mutation in the context of ALK fusions (PMID: 28676215, PMID: 27009859), is not transforming in culture but is activating in in vitro assays (PMID: 33674381, PMID: 25517749), and therefore, is predicted to lead to a gain of Alk protein function. Y
T1151R missense unknown ALK T1151R lies within the protein kinase domain and inhibitor binding region of the Alk protein (UniProt.org). T1151R has been identified in the scientific literature (PMID: 31027750), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Nov 2022).
T1343I missense unknown ALK T1343I lies within the protein kinase domain of the Alk protein (UniProt.org). T1343I is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
T429I missense unknown ALK T429I lies within the extracellular domain of the Alk protein (UniProt.org). T429I has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
T535N missense unknown ALK T535N lies within MAM domain 2 of the Alk protein (UniProt.org). T535N has been identified in sequencing studies (PMID: 34465320), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Aug 2022).
T680I missense no effect - predicted ALK T680I lies within the MAM domain 2 of the Alk protein (UniProt.org). T680I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
V1180L missense unknown ALK V1180L lies within the protein kinase domain of the Alk protein (UniProt.org). V1180L has been demonstrated to occur as a secondary resistance mutation in the context of EML4-ALK (PMID: 25228534, PMID: 27432227, PMID: 35324529), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, Nov 2022). Y
V1180X missense unknown ALK V1180X indicates any Alk missense mutation that results in replacement of the valine (V) at amino acid 1180 by a different amino acid.
V1413G missense unknown ALK V1413G lies within the cytoplasmic domain of the Alk protein (UniProt.org). V1413G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
V1545I missense no effect - predicted ALK V1545I lies within the cytoplasmic domain of the Alk protein (UniProt.org). V1545I has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
V163L missense unknown ALK V163L lies within the extracellular domain of the Alk protein (UniProt.org). V163L has been identified in sequencing studies (PMID: 24728327), but has not been biochemically characterized and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
V198M missense unknown ALK V198M lies within the extracellular domain of the Alk protein (UniProt.org). V198M has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
V476A missense no effect - predicted ALK V476A lies within the extracellular domain of the Alk protein (UniProt.org). V476A has not been biochemically characterized, but results in similar cell proliferation and viability levels to wild-type Alk in cultured cells (PMID: 29533785), and therefore, is predicted to have no effect on Alk protein function.
V66A missense unknown ALK V66A lies within the extracellular domain of the Alk protein (UniProt.org). V66A has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, Oct 2022).
V66G missense unknown ALK V66G lies within the extracellular domain of the Alk protein (UniProt.org). V66G has not been characterized in the scientific literature and therefore, its effect on Alk protein function is unknown (PubMed, May 2022).
W501* nonsense loss of function - predicted ALK W501* results in a premature truncation of the Alk protein at amino acid 501 of 1620 (UniProt.org). Due to the loss of the protein kinase domain (UniProt.org), W501* is predicted to lead to a loss of Alk protein function.
wild-type none no effect Wild-type ALK indicates that no mutation has been detected within the ALK gene.
Y1096A missense unknown ALK Y1096A lies within the cytoplasmic domain of the Alk protein (UniProt.org). Y1096A is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
Y1278A missense unknown ALK Y1278A lies within the protein kinase domain of the Alk protein (UniProt.org). Y1278A is not activating in an in vitro assay but is transforming in cultured cells (PMID: 33674381), and therefore, its effect on Alk protein function is unknown.
Y1278E missense unknown ALK Y1278E lies within the protein kinase domain of the Alk protein (UniProt.org). Y1278E is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381, PMID: 25517749), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
Y1278S missense gain of function ALK Y1278S is a hotspot mutation that lies within the activation loop of the Alk protein (PMID: 24060861, PMID: 25071110). Y1278S results in constitutive activation of Alk and is transforming in cell culture (PMID: 25517749, PMID: 29084134).
Y1282E missense unknown ALK Y1282E lies within the protein kinase domain of the Alk protein (UniProt.org). Y1282E is not activating in an in vitro assay and is not transforming in culture (PMID: 33674381), but has not been fully biochemically characterized and therefore, its effect on Alk protein function is unknown.
ARID2 - ALK fusion unknown ARID2-ALK results from the fusion of ARID2 and ALK (PMID: 35144623). ARID2-ALK has been identified in lung adenocarcinoma (PMID: 35144623), but has not been biochemically characterized and therefore, the effect on protein function is unknown (PubMed, Oct 2022).
EML4 - ALK fusion gain of function EML4-ALK results from the fusion of EML4 and ALK, resulting in constitutive kinase activity, transformation in cultured cells, and tumor formation in mouse models (PMID: 19064915, PMID: 17625570, PMID: 35138907). EML4-ALK fusions have been associated with non-small cell lung cancer (PMID: 26755435).